4 Activity 1
- Read the volumes specified on the Micropipetting Practice Card and use your micropipette to add the correct amount of liquid to each circle.
- Try to be precise so that the liquid stays in the circle.
- If you have trouble, dry the spot with a paper towel and try again.
- If you have trouble, try using both hands. You can use your non-dominant hand to stabilize the micropipette being held in her dominant hand.
- Try to pick up all of the liquid without leaving any behind.
- Set your micropipette to the same volume of liquid that is specified for each of your drops.
- Check to see if you can pick up all of the liquid without leaving any behind. There should be no space at the end of your pipette tip.
- In the blank space at the bottom of the card, pipette 5 µl of water1052 three times.
- How similar do the drops look in size?
- If they do not look exactly the same what could be some sources of error?
- On a dry place on your card place 4 µl water. Add 4 µl to the same place three more times.
- How much volume should you now have? ____[Enter data here]________
- Set your pipette to the volume you just calculated and try to pick up the entire drop.
- Was there any liquid left on the card or was there space left in the tip of your pipette? ____[Enter data here]_______
