5 Activity 2
Add the correct volume of colored liquids by pipetting precisely onto the dots of your Pipette Practice Card.
Exercises
- Add 13.5 μl of blue dye to dot A
- Add 17.5 μl of yellow dye to dot C
- Add 17 μl of red dye to dot E
- From dot A, pick up 2 μl and place it on dot F
- From dot C, pick up 4.5 μl and place it on dot B
- From dot E, pick up 3 μl and place it on dot D
Now that you have set up your card, your goal is to mix these dyes with great care and precision. To mix, you will gently pipette up and down to the micropipette’s first stop 2-3 times. Only pipette through to your micropipette’s second stop at the end to remove all liquid from your tip to avoid making any air bubbles. Follow the guidance below:
Exercises
- From dot E, pick up 6 μl and mix it with the volume already present on dot F
- From dot A, pick up 3.5 μl and mix this volume onto dot B
- From dot C, pick up 5 μl and mix this volume onto dot D
Did you make any bubbles while you were mixing?
Calculate how much volume (in units of uL) should now be on each dot:
Set your micropipette to that volume and pick up each drop one by one and move to the open space at the bottom of your card. Each time you pick up a drop, note if liquid was left behind (code: LB), or if there is any space at the end of your pipette tip (code: S).
