42 Critical Thinking Questions for Bacterial Genomic DNA Isolation
- What lab skills have you gained so far in this course that you can add to your resume? (HINT: These are listed in the Overview and Goals section of every lab protocol.)
- Read Activity 1. Why do we resuspend the pellet of bacteria before we add Lysozyme to lyse open the bacteria?
- Read Activity 1. Why do you think we keep Proteinase K on ice before we use it and then incubate it with our sample at 56°C?
- In step 3 of Activity 2, you discard the excess liquid by pipetting. This is also called… (HINT: read Activity 1).
- What future protocols inform us to isolate high molecular weight (HMW) DNA? Compare the advantage of obtaining HMW DNA compared to short fragments.
