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BIT 214 Lab Schedule
Table Of Contents
List of Protocols in Protocols.io
1. Overview and Goals
2. Preparation
3. Procedure
4. Activity 1
5. Activity 2
6. Critical Thinking Question for Pipetting
7. Overview and Goals
8. Preparation
9. Procedure
10. Clean Up Instructions
11. Critical Thinking Question for Plate Streaking
12. Overview and Goals
13. Preparation
14. Activity 1: Multichannel pipetting
15. Activity 2: Turbidimete
16. Activity 3: Biolog plate preparation
17. Clean Up Instructions
18. Critical Thinking Questions for Preparing Biological Growth Plates
19. Overview and Goals
20. Preparation
21. Procedure
22. Clean-up Instructions
23. Critical Thinking Questions Microscopy
24. Overview and Goals
25. Preparation
26. Procedure
27. Clean-Up Instructions
28. Critical Thinking Questions Microscopy
29. Overview and Goals
30. Preparation
31. Bacterial Lysis and Genomic DNA Isolation
32. Critical Thinking Questions for Bacterial Genomic DNA Isolation
33. Overview and Goals
34. Preparation
35. Bacterial Lysis and Genomic DNA Isolation
36. Critical Thinking Questions for Bacterial Genomic DNA Isolation
37. Overview and Goals
38. Preparation
39. Note Before You Begin:
40. Activity 1: Bacterial Lysis
41. Activity 2: HMW gDNA Binding and Elution
42. Critical Thinking Questions for Bacterial Genomic DNA Isolation
43. Overview and Goals
44. Preparation
45. Activity 1: Quantification of DNA with the Implen Spectrophotometer
46. Activity 2: DNA Quantification with Qubit
47. Activity 3: Genomic DNA Assessment with TapeStation
48. Critical Thinking Questions for Quantifying and Checking Genomic DNA
49. 16S Gene PCR Amplification and Sanger Sequencing
50. Preparation
51. PCR Clean-Up
52. Sanger Sequencing Sample Preparations
53. Analyzing Chromatograms to Confirm Consensus Sequence
54. Overview and Goals
55. Equipment and Consumables
56. Preparation
57. Instrument Setup and Flow Cell Priming (in parallel with sample prep)
58. Library Preparation
59. Critical Thinking Questions for Nanopore Sequencing
References
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Biotechnology and Sustainability Copyright © by Carlos Goller is licensed under a Creative Commons Attribution 4.0 International License, except where otherwise noted.