55 Equipment and Consumables
Oxford Nanopore Technologies (ONT) Kit and Flow Cells:
- ONT Rapid Barcoding Kit (RBK004)
- ONT Flow Cell Priming Kit (EXP-FLP002)
- MinION FLO-MIN106 R9.4.1 flow cells
- Sequencing auxiliary kit EXP-AUX001
All consumables:
- 1.5 mL Eppendorf DNA LoBind tubes
- 0.2 mL thin-walled PCR tubes
- Nuclease-free water (e.g., ThermoFisher, cat # AM9937)
- Freshly prepared 70% ethanol in nuclease-free water
- Qubit™ Assay Tubes (ThermoFisher Q32856)
- Qubit dsDNA HS Assay Kit (ThermoFisher Q32851)
- 10 mM Tris-HCl pH 8.0 with 50 mM NaCl (optional)
All equipment:
- ONT MinION Mk1B or MinION Mk1C and computers
- Thermal cycler or heat block at 30°C and 80°C
- Hula mixer (gentle rotator mixer)
- Magnetic separator, suitable for 1.5 ml Eppendorf tubes
- Microfuge
- Vortex mixer
- Thermal cycler
- P1000 pipette and tips
- P200 pipette and tips
- P100 pipette and tips
- P20 pipette and tips
- P10 pipette and tips
- P2.5 pipette and tips
- Ice bucket with ice
- Timer
- Qubit fluorometer (or equivalent for QC check)
Materials:
- ~400 ng of gDNA in 7.5μL of nuclease-free water
- AMPure XP Beads (AXP)
Consumables:
- 0.2 mL thin-walled PCR tubes
- 1.5 mL Eppendorf DNA LoBind tubes
