33 Overview and Goals
Bacterial Genomic DNA Isolation – QIAGEN PowerSoil Pro
Your bacterial isolate has been grown on agar plates. We now need to lyse open the bacteria and isolate only the genomic DNA for sequencing. For this, we need to lyse the bacteria with physical bead beating and a lysis buffer. We will then purify the genomic DNA. We will use the QIAGEN PowerSoil Pro DNA extraction kit.
On the left are healthy living bacteria (An example is Bacillus). On the right the bacteria are lysed open, which results in cell death. Consider this question as you read the protocol: How will we facilitate bacterial lysis? Images of Bacilli created using BioRender.com.
After completing this lab you will gain the following lab skills:
Learning Objectives
- Lab safety and proper personal protective equipment (PPE)
- Proper use of a centrifuge and pipettors
- Isolation and purification of genomic DNA
