43 Overview and Goals
Quantifying and Checking Genomic DNA
Your bacterial isolate has been grown on agar plates, lysed open, and genomic DNA has been isolated for sequencing. Next, we need to quantify our DNA yield and assess its integrity. For this, we will use the Implen spectrophotometer and Qubit fluorometer to measure our DNA quantity, We will also use an Agilent TapeStation electrophoresis system to assess the size of the DNA and quality.
After completing this lab you will gain the following lab skills:
Learning Objectives
- Lab safety and proper personal protective equipment (PPE)
- Proper use of a Implen spectrophotometer and Qubit fluorometer to analyze DNA samples.
- Analysis of DNA concentration and integrity using absorbance, fluorescence, and gel electrophoresis.
