56 Preparation
Review the “How to use a micropipette” lab we completed at the beginning of the semester. Before coming to the lab watch this 5-minute video, How to Micropipette, that demonstrates 3 key pipetting skills:
Learning Objectives
- How to hold a micropipette
- How to set a micropipette to a desired volume
- How to use the plunger and ejector
In this lab session, we will pipette small volumes of DNA and enzymes to prepare your genomic DNA for sequencing by adding adapters for the Nanopore sequencing. We will also combine all our samples once we have added adapters to bioinformatically distinguish one sample from another.
The Oxford Nanopore Technologies (ONT) Rapid Barcoding Kit will allow us to sequence the genomic DNA from several different genomic DNA samples in one flow cell by using an enzyme to cleave and add barcoded adapter sequences to the ends of the genomic DNA (Step 1). Next, sequencing adapters are added to the pooled sample (Step 2) before loading and sequencing (Step 3). Created with BioRender.com.
