25 Preparation
Visit this page to learn more about the reagents we will use: MycoLight™ rapid fluorescence bacterial Gram stain kit.
Note Before You Begin:
Please review the complete protocol before beginning. Several of the steps in this procedure are time—and/or temperature-sensitive, so it’s important that you know what to expect and how to manage your time.
We will thaw all the kit components at room temperature before use and spin down briefly. We have created single-use aliquots to avoid freeze-thaw cycles and stored them at -20°C after preparation. We prepared the MycoLight™ Red stock solution previously by adding 100 µL of ddH2O into one vial of MycoLight™ Red (Component B) and mixing well. Instructors have also prepared the MycoLight™ dye working solution by mixing equal volume of MycoLight™ Green (Component A) and MycoLight™ Red stock solution in a tube.
After completing this lab, you will gain the following lab skills:
Learning Objectives
- Lab safety and proper personal protective equipment (PPE)
- Use of Gram staining/Gram staining alternative to identify the Gram status of a bacterial isolate
- Use of a fluorescent microscope for the detection of red/green fluorescence
