50 Preparation
Note Before You Begin:
Review the protocols and figures below to learn how the 16S PCR will help us amplify regions of this gene.
PCR Table. Reagents and volumes needed for 16S PCR.
For each gDNA sample, set up two reactions:
- one LONG reaction with primers 27fwd + 1492rev
- one SHORT reaction with 515fwd + 1492rev
Primer Table. Primer names and sequences for the amplification of 16S.
Bacterial 16S Gene Schematic for Amplification and Sanger Sequencing. The structure of the 16S rRNA gene is presented along with the primers and their annealing sites. Created with BioRender.com
Thermocycler protocol table.
