9 Procedure
Each student researcher will need:
- Sharpie pen
- Kimwipes
- Sterile inoculation loops
- Pens and lab markers
- Bacterial culture source
- Luria Bertani (LB) Kanamycin and Tryptic Soy Agar (TSA) agar plates
Plate Streaking Method diagram: Created with BioRender.com
- Obtain a fresh petri dish containing TSA media.
- Label the bottom of the plate (the side containing TSA media) keeping to the circumference (write on the edge, not in the center) with your bacterial isolate#, your Team #, your initials, and today’s date. See the diagram below for a visual example. If the surface of the plate is wet, dry it off using a chem wipe.
- Obtain a sterile plastic loop.
- Inspect the plate with the bacterial isolate you will characterize.
- Using the sterile plastic loop, touch the loop to a single colony on your bacterial source plate.
- Streak the bacteria on the loop onto your labeled inoculate plate following the inoculation procedure illustrated above.
- Put your lid back on your plate, turn it upside down (lid facing the benchtop) and bring it to the incubation chamber.
- Incubate your bacterial plate overnight with the goal of obtaining single isolated colonies.
