16 Activity 3: Biolog plate preparation
Key Takeaways
1. Pour the cell suspension into the multichannel pipette reservoir.
2. Load 8 tips onto each gasket of the multichannel pipet and fill the tips by drawing up 100 μl of cell suspension from the reservoir.
3. Use the multichannel to add 100 μl of diluted bacterial culture to each of the 96 wells of the GEN III MicroPlateTM.
a. If the tips touch the wells, replace your tips before delivering inoculation fluid to the next column. Work carefully to avoid contamination!
b. Use the technique practiced in Activity 1.
4. Eject the pipettor tips if any one tip touches the wells of the plate as this can cross contamination wells with chemicals. Reload tips as needed.
5. Check that all wells have liquid
6. Cover the GEN3 microplate with its lid and eject the pipette tips.
7. Incubate at 33°C for 1-2 days. For some plates, we will incubate in a heated Agilent/Biotek LogPhase 600 plate reader and take 590 nm/600 nm readings every 15 minutes for 2 days to learn about the growth patterns.
