"

List of Protocols in Protocols.io

 

Use the Protocols.io copy of the procedure accessed to run and check off each step of the procedure.

Pipetting→

Plate Streaking → 

Preparing Biolog Growth Plates →

Bacterial Staining and Microscopy →

Bacterial Isolate Genomic DNA Isolation for NO-MISS → 

PowerSoil Pro Bacterial Genomic DNA Isolation →

Bacterial Genomic DNA Isolation →

Quantifying and Checking Genomic DNA→

Rapid Sequencing gDNA Barcoding RBK2004 (legacy) →

 

 

Biotechnology & Sustainability Research Protocols

After completing the course laboratory protocols, students can add the following skills to their research experience on their resumes:

    • Learning Objectives for BIT 214

      • Lab safety and proper personal protective equipment (PPE)
      • Proper use of equipment: Biolog plate reader, bacterial cell culture incubator, vortex, centrifuge, Implen, Miroculus Miro Canvas, Oxford Nanopore Technologies (ONT) MinION & GridION
      • Pipetting with micropipettes
      • Bacterial plate streaking
      • Bacterial plate incubation
      • High molecular weight (HMW) genomic DNA (gDNA) isolation
      • Spectrophotometer DNA quantification
      • Preparation of DNA sequencing library
      • Nanopore DNA sequencing
      • Bioinformatics analysis of high-throughput sequencing data using KBase

       

Protocol Acknowledgements and References

References to protocols & resources were adapted by Dr. Carlos Goller, Dr. Carly Sjogren, Caleb Carter, and Thomas Morgan. Dr. Carlos Goller and Dr. Carly Sjogren curated these resources for the BIT 295 Biotechnology and Sustainability course.

Lab Safety and Personal Protective Equipment

  • Before beginning lab work, watch the BIT Lab Safety video (9:40 min).
  • Note that closed-toe shoes and long pants/skirts are required for working in the laboratory space. If you arrive unprepared for this safety requirement, you will not be allowed into the laboratory and may lose access to the learning experience.
  • Eating and drinking are prohibited in laboratory spaces. You may use the carts outside the lab entry for storing drinks or snacks. You must wash your hands between bench work and consuming any food or drink, as well as when returning to the bench from consuming any food or drink.

 

License

Icon for the Creative Commons Attribution 4.0 International License

Biotechnology and Sustainability Copyright © by Carlos Goller is licensed under a Creative Commons Attribution 4.0 International License, except where otherwise noted.

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