Fall 2019: Investigating the Microbial Communities in Mortality Composts
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Methods and statistics found in selected papers.
BIT 477/577 Fall 2019 Students and Carlos Goller
Learning Objectives
- Describe what published metagenomic survey figures represent.
- Identify the main features of typical graphical representations of differences in bacterial diversity between samples.
What are some of the statistical tests that we saw in the three papers we discussed?
What statistical tests were done by QIIME2 as part of the Moving Pictures tutorial?
https://docs.qiime2.org/2019.10/tutorials/moving-pictures/#differential-abundance-testing-with-ancom
What are some tools on Microbiome Analyst that you can use?
- Taxonomic profiling
- Diversity metric box plots
- Differential abundance levels e.g. stacked bar charts
- Visualization of similarities/ dissimilarities: e.g. PCoA plot
- Functional predictions/ profiles
- Classification
- Variation between samples through clustered heatmaps
- Heatmaps: https://youtu.be/7xHsRkOdVwo and https://youtu.be/oMtDyOn2TCc