67 Characterization Techniques

Brigitte Gallagher; Jenna Diemel; and Rachel Falkowski

Fluorescence microscopy  is used to sorts cells by size and complexity based on how light reflects off the cells. this is used to determine the size of the nanoparticles that are being made and some of the nanoparticle structural topography.

Video about is Fluorescence microscopy [7].

 

Image of the inside of a fluorescence microscope [8].

Fluorescence microscopy can give 3D data including measurements, topographical images, and time lapses. Some limitations are that contracts are needed, the wavelength must be the same as the membrane that you are trying to see and it could take time to get the right wavelength.

Fluorescence helps to identify the proteins and outer layer of the nanoparticle and can record how the particle reacts to stimuli over time to give us a better understanding of how it reacts within the body.

 

 

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Polymeric Biomaterials: As Explored through TED Talks Copyright © by Wendy Krause is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, except where otherwise noted.

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