25 Scientific Paper on the Topic Written by the Speaker
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- Author’s paper
- Silk film can be used as biomaterials for corneal tissue engineering. Silk is considered to be biodegradable and biocompatible. In this paper, 2 um transparent silk film was developed with pores of 0.5 – 5.0 um. The film is capable of assisting the corneal cell functions and supports interactions among cells for regeneration of the tissue. Bombyx mori silk cocoons were prepared for the experiment through the following process: cut into 4 different sections and boiled for 45 min in Sigma-Aldrich solution to obtain sericin proteins from the silk fibroin. Silk casting was performed using the PDMS substrates to create a circular shape when the silk solution was cooled.
- Silk proteins such as the silk fibroin is very adaptive for tissue engineering and medicine application because of its characterizes of non-immunogenic response. Silk fibrin can adapt lithographic techniques for creating transparent films surfaces. Silk film developed from silk fibroin provides water-stable and transparent characteristics. Silk Film Culture System for in vitro Analysis and Biomaterial Design
- Information Provided by the Technique
- Information and data:
- Thin, optically transparent, silk film biomaterials were formed and used as a basis for culturing hCF and rCF cells.
- Both flat and patterned silk film surfaces showed increased cell growth over time. The only difference was the cellular morphology.
- Application of actin filament influenced the presence of surface patterning. Presence of the filament resulted in increased cellular alignment of along the applied pattern.
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- More research needs to be done to investigate the long-term matrix generation.
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- Importance of Silk Film
- Biodegradable: Controllable biodegradation rates are possible through silk fibroin. This allows extended native tissue remodeling time. Other materials such as synthetic polyglycolic acid, or natural collagen, degrade more rapidly or would require crosslinking.
- Sustainable: Regenerated silk fibroin has shown to have reduced bioburden content. This is an advantage as corneal tissue engineering generally apply acellular allograft tissue (bioburden content), which requires an animal source.
- Implantable: Patterned silk film provided adequate conditions to control cell alignment and promote native extracellular matrix (ECM) synthesis. The silk material is able to emulate the layered and aligned structures that form in native cornea.
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